Recombinant human Siglec-3/CD33 protein
CD33 is putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. This protein induces apoptosis in acute myeloid leukemia (in vitro) . Recombinant human CD33 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.1 kDa (265aa)
Additionnal Information
References & Citations
Ulyanova T., et al. (1999) Eur. J. Immunol. 29:3440-3449; ; Vitale C., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:5764-5769;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Stem cell
NCBI Accession Number
AAH28152
Uniprot Accession Number
P20138
Species
Human
AA Sequence
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