Recombinant human MREG protein

Melanoregulin, also known as MREG, is thought to play a role in membrane fusion and in regulating the biogenesis of disk membranes of photoreceptor rods. MREG is expressed in photoreceptor cells as two isoforms due to alternative splicing. MREG interacts with Peripherin-2, a photoreceptor specific tetraspanin protein that is required to maintain normal cell structure during the renewal process of membrane fusion. Recombinant human MREG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Melanoregulin, DSu, WDT2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.3 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

MREG, Melanoregulin, DSu, WDT2, ATGP2582-10 µg, ATGP2582-20 µg, ATGP2582-50 µg, ATGP2582-100 µg, ATGP2582-250 µg, ATGP2582-500 µg, ATGP2582-1 mg, ATGP2582-10, ATGP2582-20, ATGP2582-50, ATGP2582-100, ATGP2582-250, ATGP2582-500, ATGP2582-1

References & Citations

Loewen C J., et al. (2003) Mol Biol Cell. 14: 3400-3413.; ; Roof D J., et al. (1982) J Cell Biol. 95: 501-509.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Other

NCBI Accession Number

NP_060470.2

Uniprot Accession Number

Q8N565

Species

Human

AA Sequence

MGLRDWL RTVCCCCGCE CLEERALPEK EPLVSDNNPY SSFGATLVRD DEKNLWSMPH DVSHTEADDD RTLYNLIVIR NQQAKDSEEW QKLNYDIHTL RQVRREVRNR WKCILEDLGF QKEADSLLSV TKLSTISDSK NTRKAREMLL KLAEETNIFP TSWELSERYL FVVDRLIALD AAEEFFKLAR RTYPKKPGVP CLADGQKELH YLPFPSP