Recombinant human HPR protein

HPR, also known as Haptoglobin-related protein, is a primate-specific plasma protein associated with apolipoprotein L-I (apoL-I) -containing high-density lipoprotein (HDL) particles shown to be a part of the innate immune defense. HPR-bound hemoglobin might contribute to the biologic activity of the circulating apoL-I/Hpr-containing HDL particles. Recombinant human HPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Haptoglobin-related protein, A-259H10.2, HP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

39.3 kDa (352aa) confirmed by MALDI-TOF

Additionnal Information

HPR, Haptoglobin-related protein, A-259H10.2, HP, ATGP2581-10 µg, ATGP2581-20 µg, ATGP2581-50 µg, ATGP2581-100 µg, ATGP2581-250 µg, ATGP2581-500 µg, ATGP2581-1 mg, ATGP2581-10, ATGP2581-20, ATGP2581-50, ATGP2581-100, ATGP2581-250, ATGP2581-500, ATGP2581-1

References & Citations

Nielsen MJ. et al. (2008) Blood. 108:2846-2849.; ; Imrie HJ. et al. (2012) PLoS One. 7:e49816;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 20% glycerol, 1mM DTT, 0.15M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_066275

Uniprot Accession Number

P00739

Species

Human

AA Sequence

LYSGNDV TDISDDRFPK PPEIANGYVE HLFRYQCKNY YRLRTEGDGV YTLNDKKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYHQ VDIGLIKLKQ KVLVNERVMP ICLPSKNYAE VGRVGYVSGW GQSDNFKLTD HLKYVMLPVA DQYDCITHYE GSTCPKWKAP KSPVGVQPIL NEHTFCVGMS KYQEDTCYGD AGSAFAVHDL EEDTWYAAGI LSFDKSCAVA EYGVYVKVTS IQHWVQKTIA EN