Recombinant human CD45 protein

PTPRC, also known as CD45, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Recombinant human PTPRC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein tyrosine phosphatase receptor type isoform 1, Protein tyrosine phosphatase, receptor type, isoform 1, LCA, LY5, B220, CD45, T200, GP180

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.6 kDa (257aa) confirmed by MALDI-TOF

Additionnal Information

CD45, Protein tyrosine phosphatase receptor type isoform 1, Protein tyrosine phosphatase, receptor type, isoform 1, LCA, LY5, B220, CD45, T200, GP180, ATGP2577-10 µg, ATGP2577-20 µg, ATGP2577-50 µg, ATGP2577-100 µg, ATGP2577-250 µg, ATGP2577-500 µg, ATGP2577-1 mg, ATGP2577-10, ATGP2577-20, ATGP2577-50, ATGP2577-100, ATGP2577-250, ATGP2577-500, ATGP2577-1

References & Citations

Charbonneau H., et al. (1988) Proc. Natl. Acad. Sci. u.S.A. 85:7182-7186; ; Wu L., et al. (2002) Mol. Cell. Biol. 22:2673-2686;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Stem cell

NCBI Accession Number

NP_002829.3

Uniprot Accession Number

P08575

Species

Human

AA Sequence

VMIA AQGPLKETIG DFWQMIFQRK VKVIVMLTEL KHGDQEICAQ YWGEGKQTYG DIEVDLKDTD KSSTYTLRVF ELRHSKRKDS RTVYQYQYTN WSVEQLPAEP KELISMIQVV KQKLPQKNSS EGNKHHKSTP LLIHCRDGSQ QTGIFCALLN LLESAETEEV VDIFQVVKAL RKARPGMVST FEQYQFLYDV IASTYPAQNG QVKKNNHQED KIEFDNE