Recombinant human CHRNA6 protein

Cholinergic receptor, nicotinic, alpha 6, also known as CHRNA6, belongs to the nicotinic acetylcholine receptors (nAChRs) family. nAChRs are heteropentameric ligand-gated ion channels resulting from the assembly of alpha and beta protein subunits. This ion channel is gated by the binding of acetylcholine to the assembled receptor. Alpha 6 is often found associated with beta 3, beta 2, and alpha 4 subunits. CHRNA6 receptors have a relatively selective localization to the nigrostriatal pathway where they function to mediate dopamine release. Recombinant human CHRNA6 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cholinergic receptor nicotinic alpha, Cholinergic receptor, nicotinic, alpha, CHNRA6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.3 kDa (250aa)

Additionnal Information

CHRNA6, Cholinergic receptor nicotinic alpha, Cholinergic receptor, nicotinic, alpha, CHNRA6, ATGP2572-10 µg, ATGP2572-20 µg, ATGP2572-50 µg, ATGP2572-100 µg, ATGP2572-250 µg, ATGP2572-500 µg, ATGP2572-1 mg, ATGP2572-10, ATGP2572-20, ATGP2572-50, ATGP2572-100, ATGP2572-250, ATGP2572-500, ATGP2572-1

References & Citations

Keskitalo Vuokko k., et al. (2011) Nicotine Tob Res. 13 (8) : 686-90.; ; Groot Kormrlink P J., et al. (2004) J Gen Physiol. 123: 697-708.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_004189

Uniprot Accession Number

Q15825

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSKGCV GCATEERLFH KLFSHYNQFI RPVENVSDPV TVHFEVAITQ LANVDEVNQI METNLWLRHI WNDYKLRWDP MEYDGIETLR VPADKIWKPD IVLYNNAVGD FQVEGKTKAL LKYNGMITWT PPAIFKSSCP MDITFFPFDH QNCSLKFGSW TYDKAEIDLL IIGSKVDMND FWENSEWEII DASGYKHDIK YNCCEEIYTD ITYSFYIRRL