Recombinant human Heparanase/HPSE protein
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. HPSE is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Recombinant human HPSE protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Heparanase isoform 1 preproprotein, Heparanase, HPA, HPA1, HPR1, HPSE1, HSE1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
60 kDa (531aa)
Additionnal Information
References & Citations
Okada Y., et al. (2002) J. Biol. Chem. 277:42488-42495.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Cancer
NCBI Accession Number
NP_006656
Uniprot Accession Number
Q9Y251
Species
Human
AA Sequence
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