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Recombinant human Heparanase/HPSE protein

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. HPSE is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Recombinant human HPSE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Heparanase isoform 1 preproprotein, Heparanase, HPA, HPA1, HPR1, HPSE1, HSE1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

60 kDa (531aa)

Additionnal Information

HPSE, Heparanase isoform 1 preproprotein, Heparanase, HPA, HPA1, HPR1, HPSE1, HSE1, ATGP2564-10 µg, ATGP2564-20 µg, ATGP2564-50 µg, ATGP2564-100 µg, ATGP2564-250 µg, ATGP2564-500 µg, ATGP2564-1 mg, ATGP2564-10, ATGP2564-20, ATGP2564-50, ATGP2564-100, ATGP2564-250, ATGP2564-500, ATGP2564-1

References & Citations

Okada Y., et al. (2002) J. Biol. Chem. 277:42488-42495.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Cancer

NCBI Accession Number

NP_006656

Uniprot Accession Number

Q9Y251

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I

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