Recombinant human Epoxide Hydrolase 1/EPHX1 protein

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Recombinant human EPHX1 protein, fused to T7-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Epoxide hydrolase 1, Epoxide hydrolase 1 microsomal (xenobiotic), MEH, EPHX, EPOX

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.2 kDa (451aa)

Additionnal Information

Microsomal (xenobiotic), MEH, Epoxide hydrolase 1 microsomal (xenobiotic), Epoxide hydrolase 1, EPOX, EPHX1, EPHX, ATGP2560-10 µg, ATGP2560-20 µg, ATGP2560-50 µg, ATGP2560-100 µg, ATGP2560-250 µg, ATGP2560-500 µg, ATGP2560-1 mg, ATGP2560-010, ATGP2560-020, ATGP2560-050, ATGP2560-100, ATGP2560-250, ATGP2560-500, ATGP2560-01M

References & Citations

Nguyen HL, et al. RNA, (2013) . PMID 23564882.; ; Wang S, et al. Tumour Biol, (2013) . PMID 23378225.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_000111

Uniprot Accession Number

P07099

Species

Human

AA Sequence

MASMTGGQQM GRGSHMRDKE ETLPLEDGWW GPGTRSAARE DDSIRPFKVE TSDEEIHDLH QRIDKFRFTP PLEDSCFHYG FNSNYLKKVI SYWRNEFDWK KQVEILNRYP HFKTKIEGLD IHFIHVKPPQ LPAGHTPKPL LMVHGWPGSF YEFYKIIPLL TDPKNHGLSD EHVFEVICPS IPGYGFSEAS SKKGFNSVAT ARIFYKLMLR LGFQEFYIQG GDWGSLICTN MAQLVPSHVK GLHLNMALVL SNFSTLTLLL GQRFGRFLGL TERDVELLYP VKEKVFYSLM RESGYMHIQC TKPDTVGSAL NDSPVGLAAY ILEKFSTWTN TEFRYLEDGG LERKFSLDDL LTNVMLYWTT GTIISSQRFY KENLGQGWMT QKHERMKVYV PTGFSAFPFE LLHTPEKWVR FKYPKLISYS YMVRGGHFAA FEEPELLAQD IRKFLSVLER Q