Recombinant human GET4 protein

GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Recombinant human GET4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Golgi to ER traffic protein 4 homolog, Golgi to ER traffic protein 4 homolog (S. cerevisiae), C7orf20, CEE, CGI-20, TRC35

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.9 kDa (350aa)

Additionnal Information

GET4, Golgi to ER traffic protein 4 homolog, Golgi to ER traffic protein 4 homolog (S. cerevisiae), C7orf20, CEE, CGI-20, TRC35, ATGP2559-10 µg, ATGP2559-20 µg, ATGP2559-50 µg, ATGP2559-100 µg, ATGP2559-250 µg, ATGP2559-500 µg, ATGP2559-1 mg, ATGP2559-10, ATGP2559-20, ATGP2559-50, ATGP2559-100, ATGP2559-250, ATGP2559-500, ATGP2559-1

References & Citations

Mariappan M., Li X., et al. (2010) Nature 466:1120-1124.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Other

NCBI Accession Number

NP_057033

Uniprot Accession Number

Q7L5D6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD