Recombinant human USP46/UAF1 protein

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. uSP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. Recombinant human uSP46 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 46 isoform 1, Deubiquitinating enzyme 46, FLJ12552, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin thioesterase 46, ubiquitin-specific-processing protease 46

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BRADFORD assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.8 kDa (389aa) confirmed by MALDI-TOF

Additionnal Information

USP46, UAF1, ubiquitin carboxyl-terminal hydrolase 46 isoform 1, Deubiquitinating enzyme 46, FLJ12552, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin thioesterase 46, ubiquitin-specific-processing protease 46, ATGP2553-10 µg, ATGP2553-20 µg, ATGP2553-50 µg, ATGP2553-100 µg, ATGP2553-250 µg, ATGP2553-500 µg, ATGP2553-1 mg, ATGP2553-10, ATGP2553-20, ATGP2553-50, ATGP2553-100, ATGP2553-250, ATGP2553-500, ATGP2553-1

References & Citations

Fukuo, Y., et al. (2011) J Affect Disord 133 (1-2), 150-157; ; Zhang, W., et al. (2011) PLoS ONE 6 (10), E26297;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_073743.2

Uniprot Accession Number

P62068

Species

Human

AA Sequence

MTVRNIA SICNMGTNAS ALEKDIGPEQ FPINEHYFGL VNFGNTCYCN SVLQALYFCR PFRENVLAYK AQQKKKENLL TCLADLFHSI ATQKKKVGVI PPKKFISRLR KENDLFDNYM QQDAHEFLNY LLNTIADILQ EEKKQEKQNG KLKNGNMNEP AENNKPELTW VHEIFQGTLT NETRCLNCET VSSKDEDFLD LSVDVEQNTS ITHCLRDFSN TETLCSEQKY YCETCCSKQE AQKRMRVKKL PMILALHLKR FKYMEQLHRY TKLSYRVVFP LELRLFNTSS DAVNLDRMYD LVAVVVHCGS GPNRGHYITI VKSHGFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRE