Recombinant human KLF6 protein

KLF6, also known as Krueppel-like factor 6, is a nuclear protein that has three zinc fingers. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region might be involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain might play an important role in the process of transcriptional activation. It is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. Recombinant human KLF6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Kruppel-like factor 6, Kruppel-like factor 6, B-cell-derived protein 1, BCD1, CBA1, COPEB, CPBP, Kruppel like factor 6, core promoter element binding protein, GBF, GC-rich binding factor, PAC1, Zf9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.3 kDa (306aa) confirmed by MALDI-TOF

Additionnal Information

KLF6, ATGP2550-10 µg, ATGP2550-20 µg, ATGP2550-50 µg, ATGP2550-100 µg, ATGP2550-250 µg, ATGP2550-500 µg, ATGP2550-1 mg, ATGP2550-10, ATGP2550-20, ATGP2550-50, ATGP2550-100, ATGP2550-250, ATGP2550-500, ATGP2550-1

References & Citations

Narla G. et al. (2003) Am J Pathol. 162:1047-1052.; ; Narla G. et al. (2001) Science. 294:2563-2566;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Stem cell

NCBI Accession Number

NP_001291

Uniprot Accession Number

Q99612

Species

Human

AA Sequence

MDVLPMC SIFQELQIVH ETGYFSALPS LEEYWQQTCL ELERYLQSEP CYVSASEIKF DSQEDLWTKI ILAREKKEES ELKISSSPPE DTLISPSFCY NLETNSLNSD VSSESSDSSE ELSPTAKFTS DPIGEVLVSS GKLSSSVTST PPSSPELSRE PSQLWGCVPG ELPSPGKVRS GTSGKPGDKG NGDASPDGRR RVHRCHFNGC RKVYTKSSHL KAHQRTHTGE KPYRCSWEGC EWRFARSDEL TRHFRKHTGA KPFKCSHCDR CFSRSDHLAL HMKRHL