Recombinant human KIBRA/WWC1 protein
WWC1 is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of WWC1 have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human WWC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
17 kDa (152aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Buether K., Plaas C., et al. (2004) Biochem. Biophys. Res. Commun. 317:703-707
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 1mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_056053.1
Uniprot Accession Number
Q8IX03
Species
Human
AA Sequence
EAVGATR IQIALKYDEK NKQFAILIIQ LSNLSALLQQ QDQKVNIRVA VLPCSESTTC LFRTRPLDAS DTLVFNEVFW VSMSYPALHQ KTLRVDVCTT DRSHLEECLG GAQISLAEVC RSGERSTRWY NL
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