Recombinant human CRTAP protein

Cartilage-associated protein precursor, also known as CRTAP, is a secreted protein localizing to the extracellular space that plays a role in collagen post-translational modifications, extracellular fibril assembly and intracellular trafficking. CRTAP is widely expressed with predominant expression in articular chondrocytes. Mutations in the gene encoding CRTAP can lead to autosomal recessive osteogenesis imperfecta (OI) type 7 and type 2B. OI, also known as brittle bone disease, is characterized by bone fragility and susceptibility to fractures. Recombinant human CRTAP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cartilage-associated protein precursor, Cartilage-associated protein precursor, CASP, LEPREL3, OI7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.4 kDa (398aa)

Additionnal Information

CRTAP, Cartilage-associated protein precursor, Cartilage-associated protein precursor, CASP, LEPREL3, OI7, ATGP2548-10 µg, ATGP2548-20 µg, ATGP2548-50 µg, ATGP2548-100 µg, ATGP2548-250 µg, ATGP2548-500 µg, ATGP2548-1 mg, ATGP2548-10, ATGP2548-20, ATGP2548-50, ATGP2548-100, ATGP2548-250, ATGP2548-500, ATGP2548-1

References & Citations

Tonachini L., et al. (1999) Cytogenet Cell Genet. 87: 191-194.; ; Castagnola P., et al. (1997) J Cell Sci. 110: 1351-1359.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006362

Uniprot Accession Number

O75718

Species

Human

AA Sequence

QYERYSF RSFPRDELMP LESAYRHALD KYSGEHWAES VGYLEISLRL HRLLRDSEAF CHRNCSAAPQ PEPAAGLASY PELRLFGGLL RRAHCLKRCK QGLPAFRQSQ PSREVLADFQ RREPYKFLQF AYFKANNLPK AIAAAHTFLL KHPDDEMMKR NMAYYKSLPG AEDYIKDLET KSYESLFIRA VRAYNGENWR TSITDMELAL PDFFKAFYEC LAACEGSREI KDFKDFYLSI ADHYVEVLEC KIQCEENLTP VIGGYPVEKF VATMYHYLQF AYYKLNDLKN AAPCAVSYLL FDQNDKVMQQ NLVYYQYHRD TWGLSDEHFQ PRPEAVQFFN VTTLQKELYD FAKENIMDDD EGEVVEYVDD LLELEETS