Recombinant human RAD51D protein

RAD51D is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Recombinant human RAD51D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RAD51 homolog D isoform 1, RAD51 homolog D isoform 1, BROVCA4, R51H3, RAD51L3, TRAD

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.4 kDa (351aa) confirmed by MALDI-TOF

Additionnal Information

RAD51D, RAD51 homolog D isoform 1, RAD51 homolog D isoform 1, BROVCA4, R51H3, RAD51L3, TRAD, ATGP2545-10 µg, ATGP2545-20 µg, ATGP2545-50 µg, ATGP2545-100 µg, ATGP2545-250 µg, ATGP2545-500 µg, ATGP2545-1 mg, ATGP2545-10, ATGP2545-20, ATGP2545-50, ATGP2545-100, ATGP2545-250, ATGP2545-500, ATGP2545-1

References & Citations

Masson J.Y., et al. (2001) Genes Dev. 15:3296-3307; ; Liu N., et al. (2002) Nucleic Acids Res. 30:1009-1015;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002869

Uniprot Accession Number

O75771

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T