Recombinant human MMP-8 protein

MMP8 protein of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. MMP8 is part of a cluster of MMP. Recombinant human MMP8 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Neutrophil collagenase preproprotein, CLG1, HNC, MMP-8, PMNL-CL, Matrix metalloproteinase-8, MMP-8, PMNL collagenase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

44.3 kDa (390aa)

Additionnal Information

MMP8, neutrophil collagenase preproprotein, CLG1, HNC, MMP-8, PMNL-CL, ATGP2543-10 µg, ATGP2543-20 µg, ATGP2543-50 µg, ATGP2543-100 µg, ATGP2543-250 µg, ATGP2543-500 µg, ATGP2543-1 mg, ATGP2543-10, ATGP2543-20, ATGP2543-50, ATGP2543-100, ATGP2543-250, ATGP2543-500, ATGP2543-1

References & Citations

Hasty K.A., et al (1990) . J. Biol. Chem. 265:11421-11424; ; Betz M., et al (1997) . Eur. J. Biochem. 247:356-363;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Cancer

NCBI Accession Number

NP_002415.1

Uniprot Accession Number

P22894

Species

Human

AA Sequence

LTPGNPK WERTNLTYRI RNYTPQLSEA EVERAIKDAF ELWSVASPLI FTRISQGEAD INIAFYQRDH GDNSPFDGPN GILAHAFQPG QGIGGDAHFD AEETWTNTSA NYNLFLVAAH EFGHSLGLAH SSDPGALMYP NYAFRETSNY SLPQDDIDGI QAIYGLSSNP IQPTGPSTPK PCDPSLTFDA ITTLRGEILF FKDRYFWRRH PQLQRVEMNF ISLFWPSLPT GIQAAYEDFD RDLIFLFKGN QYWALSGYDI LQGYPKDISN YGFPSSVQAI DAAVFYRSKT YFFVNDQFWR YDNQRQFMEP GYPKSISGAF PGIESKVDAV FQQEHFFHVF SGPRYYAFDL IAQRVTRVAR GNKWLNCRYG