Recombinant human MMP-13 protein

MMP13 of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Recombinant human MMP13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Collagenase 3 preproprotein, collagenase 3 preproprotein, CLG3, MANDP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 85% by SDS-PAGE

Molecular Weight

44.7 kDa (391aa) confirmed by MALDI-TOF

Additionnal Information

MMP13, collagenase 3 preproprotein, collagenase 3 preproprotein, CLG3, MANDP1, ATGP2540-10 µg, ATGP2540-20 µg, ATGP2540-50 µg, ATGP2540-100 µg, ATGP2540-250 µg, ATGP2540-500 µg, ATGP2540-1 mg, ATGP2540-10, ATGP2540-20, ATGP2540-50, ATGP2540-100, ATGP2540-250, ATGP2540-500, ATGP2540-1

References & Citations

Gomis-Rueth F.-X., et al (1996) . J. Mol. Biol. 264:556-566; ; Lausch E., et al (2009) . Am. J. Hum. Genet. 85:168-178;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002418.1

Uniprot Accession Number

P45452

Species

Human

AA Sequence

YNVFPRT LKWSKMNLTY RIVNYTPDMT HSEVEKAFKK AFKVWSDVTP LNFTRLHDGI ADIMISFGIK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS HFMLPDDDVQ GIQSLYGPGD EDPNPKHPKT PDKCDPSLSL DAITSLRGET MIFKDRFFWR LHPQQVDAEL FLTKSFWPEL PNRIDAAYEH PSHDLIFIFR GRKFWALNGY DILEGYPKKI SELGLPKEVK KISAAVHFED TGKTLLFSGN QVWRYDDTNH IMDKDYPRLI EEDFPGIGDK VDAVYEKNGY IYFFNGPIQF EYSIWSNRIV RVMPANSILW C