Recombinant human MT3 protein
Metallothionein 3, also known as MT3, binds heavy metals. This protein contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. This protein inhibits survival and neurite formation of cortical neurons in vitro. Recombinant human MT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Metallothionein 3, Metallothionein 3, GIF, GIFB, GRIF, ZnMT3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
9.3 kDa (91aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Uchida Y., et al. (1991) Neuron. 7:337-347; ; Palmiter R.D., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:6333-6337;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_005945.1
Uniprot Accession Number
P25713
Species
Human
AA Sequence
MDPETCP CPSGGSCTCA DSCKCEGCKC TSCKKSCCSC CPAECEKCAK DCVCKGGEAA EAEAEKCSCC Q
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