Recombinant human EAF1 protein
EAF1 belongs to the EAF family and is strongly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine and colon. This protein acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Recombinant human EAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ELL associated factor 1, ELL-associated factor 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by BCA assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
11.3 kDa (110aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Simone F., et al. (2001) Blood. 98:201-209; ; Kong S.E., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102:10094-10098;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_149074.3
Uniprot Accession Number
Q96JC9
Species
Human
AA Sequence
DIIEQMS SSSGSSSSDS ESSSGSDDDS SSSGGEDNGP ASPPQPSHQQ PYNSRPAVAN GTSRPQGSNQ LMNTLRNDLQ LSESGSDSDD
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