Recombinant human PDLIM1 protein

PDLIM1 is a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. Recombinant human PDLIM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PDZ and LIM domain protein 1, CLIM1, CLP-36, CLP36, Hclim1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.7 kDa (354aa) confirmed by MALDI-TOF

Additionnal Information

PDLIM1, PDZ and LIM domain protein 1, CLIM1, CLP-36, CLP36, Hclim1, ATGP2527-10 µg, ATGP2527-20 µg, ATGP2527-50 µg, ATGP2527-100 µg, ATGP2527-250 µg, ATGP2527-500 µg, ATGP2527-1 mg, ATGP2527-10, ATGP2527-20, ATGP2527-50, ATGP2527-100, ATGP2527-250, ATGP2527-500, ATGP2527-1

References & Citations

Bauer K., et al. (2000) Blood. 96: 4236-4245.; ; Vallenius T., et al. (2002) J Cell Sci. 115: 2067-2073.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_066272

Uniprot Accession Number

O00151

Species

Human

AA Sequence

MTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK

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