Recombinant human MCM7 protein

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Recombinant human MCM7 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

48.6 kDa (437aa)

Additionnal Information

MCM7, DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, ATGP2524-10 µg, ATGP2524-20 µg, ATGP2524-50 µg, ATGP2524-100 µg, ATGP2524-250 µg, ATGP2524-500 µg, ATGP2524-1 mg, ATGP2524-10, ATGP2524-20, ATGP2524-50, ATGP2524-100, ATGP2524-250, ATGP2524-500, ATGP2524-1

References & Citations

Zhou, Y.M., et al. (2012) Liver Int. 32 (10), 1505-1509; ; Liu, Y.Z., et al. (2012) Lung Cancer 77 (1), 176-182;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_877577

Uniprot Accession Number

P33993

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMVVATYT CDQCGAETYQ PIQSPTFMPL IMCPSQECQT NRSGGRLYLQ TRGSRFIKFQ EMKMQEHSDQ VPVGNIPRSI TVLVEGENTR IAQPGDHVSV TGIFLPILRT GFRQVVQGLL SETYLEAHRI VKMNKSEDDE SGAGELTREE LRQIAEEDFY EKLAASIAPE IYGHEDVKKA LLLLLVGGVD QSPRGMKIRG NINICLMGDP GVAKSQLLSY IDRLAPRSQY TTGRGSSGVG LTAAVLRDSV SGELTLEGGA LVLADQGVCC IDEFDKMAEA DRTAIHEVME QQTISIAKAG ILTTLNARCS ILAAANPAYG RYNPRRSLEQ NIQLPAALLS RFDLLWLIQD RPDRDNDLRL AQHITYVHQH SRQPPSQFEP LDMKLMRRYI AMCREKQPMV PESLADYITA AYVEMRR