Recombinant human ATP5F1 protein
ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . Recombinant human ATP5F1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
ATP synthase subunit b, PIG47
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
22.6 kDa (197aa)
Additionnal Information
References & Citations
Higuti T., et al. (1991) Biochem. Biophys. Res. Commun. 178:1014-1020; ; Choudhary C., et al. (2009) Science. 325:834-840;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001679
Uniprot Accession Number
P24539
Species
Human
AA Sequence
Frequently Asked Questions
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