Recombinant human ATP5F1 protein

ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . Recombinant human ATP5F1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

ATP synthase subunit b, PIG47

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

22.6 kDa (197aa)

Additionnal Information

ATP5F1, ATP synthase subunit b, PIG47, ATGP2516-10 µg, ATGP2516-20 µg, ATGP2516-50 µg, ATGP2516-100 µg, ATGP2516-250 µg, ATGP2516-500 µg, ATGP2516-1 mg, ATGP2516-10, ATGP2516-20, ATGP2516-50, ATGP2516-100, ATGP2516-250, ATGP2516-500, ATGP2516-1

References & Citations

Higuti T., et al. (1991) Biochem. Biophys. Res. Commun. 178:1014-1020; ; Choudhary C., et al. (2009) Science. 325:834-840;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001679

Uniprot Accession Number

P24539

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM