Recombinant human LY6D protein
LY6D contains 1 uPAR/Ly6 domain and is expressed exclusively at the outer cell surface of transitional epithelia and the keratinocyte of stratified squamous epithelia. This protein may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification. Recombinant human LY6D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Lymphocyte antigen 6D precursor, E48, Ly-6D, Lymphocyte antigen 6 family member D, Lymphocyte antigen 6 complex, Locus D
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
10.8 kDa (101aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Inlay MA, Bhattacharya D et al. (2009) . Genes Dev. 23 (20) :2376-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol
Scientific Category
Stem cell
NCBI Accession Number
NP_003686
Uniprot Accession Number
Q14210
Species
Human
AA Sequence
LRCHVCT SSSNCKHSVV CPASSRFCKT TNTVEPLRGN LVKKDCAESC TPSYTLQGQV SSGTSSTQCC QEDLCNEKLH N
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