Recombinant human BRK1 protein
BRK1 involved in regulation of actin and microtubule organization. This protein is a part of a WAVE complex that activates the Arp2/3 complex. It belongs to the BRK1 family. Recombinant human BRK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
11.1 kDa (98aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Eden S, Rohatgi R, et al. (2002) . Nature. 418 (6899) :790-3.; ; Cascon A, Escobar B, et al. (2007) . Hum Mutat. 28 (6) :613-21.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_060932
Uniprot Accession Number
Q8WUW1
Species
Human
AA Sequence
MAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT
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