Recombinant human CKS1B protein
CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. Recombinant human CKS1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cyclin-dependent kinases regulatory subunit 1, CDC28 protein kinase regulatory subunit 1B, CKS1, ckshs1, PNAS-16, PNAS-18
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
12.0 kDa (102aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Richardson H.E., Stueland C.S., et al. (1990) Genes Dev. 4:1332-1344
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_001817
Uniprot Accession Number
P61024
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSHKQIY YSDKYDDEEF EYRHVMLPKD IAKLVPKTHL MSESEWRNLG VQQSQGWVHY MIHEPEPHIL LFRRPLPKKP KK
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