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Recombinant human CKS1B protein

CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. Recombinant human CKS1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cyclin-dependent kinases regulatory subunit 1, CDC28 protein kinase regulatory subunit 1B, CKS1, ckshs1, PNAS-16, PNAS-18

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

12.0 kDa (102aa) confirmed by MALDI-TOF

Additionnal Information

CKS1B, Cyclin-dependent kinases regulatory subunit 1, CDC28 protein kinase regulatory subunit 1B, CKS1, ckshs1, PNAS-16, PNAS-18, ATGP2511-10 µg, ATGP2511-20 µg, ATGP2511-50 µg, ATGP2511-100 µg, ATGP2511-250 µg, ATGP2511-500 µg, ATGP2511-1 mg, ATGP2511-10, ATGP2511-20, ATGP2511-50, ATGP2511-100, ATGP2511-250, ATGP2511-500, ATGP2511-1

References & Citations

Richardson H.E., Stueland C.S., et al. (1990) Genes Dev. 4:1332-1344

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001817

Uniprot Accession Number

P61024

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSHKQIY YSDKYDDEEF EYRHVMLPKD IAKLVPKTHL MSESEWRNLG VQQSQGWVHY MIHEPEPHIL LFRRPLPKKP KK

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