Recombinant human GSK-3 beta protein

GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Recombinant human GSK3B protein, was expressed in E. coli.

Product Specifications

Product Name Alternative

Glycogen synthase kinase 3 beta isoform 2, GSK-3 beta, Glycogen synthase kinase-3 beta

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46 kDa (420aa)

Additionnal Information

GSK3 b, Glycogen synthase kinase 3 beta isoform 2, GSK-3 beta, Glycogen synthase kinase-3 beta, ATGP2503-10 µg, ATGP2503-20 µg, ATGP2503-50 µg, ATGP2503-100 µg, ATGP2503-250 µg, ATGP2503-500 µg, ATGP2503-1 mg, ATGP2503-10, ATGP2503-20, ATGP2503-50, ATGP2503-100, ATGP2503-250, ATGP2503-500, ATGP2503-1

References & Citations

Guo X, Ramirez A, et al. (2008) . Genes Dev. 22 (1) :106-20.; ; Espinosa L, Ingles-Esteve J, et al. (2003) . J Biol Chem. 278 (34) :32227-35.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001139628

Uniprot Accession Number

P49841

Species

Human

AA Sequence

MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA NTGDRGQTNN AASASASNST