Recombinant human BRMS1 protein

BRMS1 reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC) . The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. Recombinant human BRMS1 protein, fused to T7-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Breast cancer metastasis suppressor 1 isoform 1, Breast cancer metastasis suppressor 1, DKFZp564A063

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

29.9 kDa (261aa)

Additionnal Information

BRMS1, Breast cancer metastasis suppressor 1 isoform 1, Breast cancer metastasis suppressor 1, DKFZp564A063, ATGP2502-10 µg, ATGP2502-20 µg, ATGP2502-50 µg, ATGP2502-100 µg, ATGP2502-250 µg, ATGP2502-500 µg, ATGP2502-1 mg, ATGP2502-10, ATGP2502-20, ATGP2502-50, ATGP2502-100, ATGP2502-250, ATGP2502-500, ATGP2502-1

References & Citations

Meehan W.J., et al. (2004) J. Biol. Chem. 279:1562-1569.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_056214.1

Uniprot Accession Number

Q9HCU9

Species

Human

AA Sequence

MASMTGGQQM GRGSHMPVQP PSKDTEEMEA EGDSAAEMNG EEEESEEERS GSQTESEEES SEMDDEDYER RRSECVSEML DLEKQFSELK EKLFRERLSQ LRLRLEEVGA ERAPEYTEPL GGLQRSLKIR IQVAGIYKGF CLDVIRNKYE CELQGAKQHL ESEKLLLYDT LQGELQERIQ RLEEDRQSLD LSSEWWDDKL HARGSSRSWD SLPPSKRKKA PLVSGPYIVY MLQEIDILED WTAIKKARAA VSPQKRKSDG P