Recombinant human MIA protein
MIA belongs to the MIA/OTOR family and contains 1 SH3 domain. This protein elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Recombinant human MIA protein, fused to His-tag at N-terminus, was expressed in E. coli
Product Specifications
Product Name Alternative
Melanoma inhibitory activity, CD-RAP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.4 kDa (128aa)
Additionnal Information
References & Citations
Blesch A, Bosserhoff AK, et al. (1994) Cancer Res 54 (21) : 5695-701.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Cancer
NCBI Accession Number
NP_006524.1
Uniprot Accession Number
Q16674
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
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