Recombinant human CCM2 protein

CCM2 provides instructions for making a protein called malcavernin, which strengthens the interactions between cells that form blood vessels and limits leakage from the vessels. It interacts with a number of other proteins to form a complex that is found in the junctions that connect neighboring cells. It helps turn off (suppress) a signaling molecule known as RhoA-GTPase. Recombinant human CCM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Malcavernin isoform 4, Malcavernin isoform 4, Malcavernin isoform 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.3 kDa (311aa) confirmed by MALDI-TOF

Additionnal Information

CCM2, Malcavernin isoform 4, Malcavernin isoform 4, Malcavernin isoform 4, ATGP2496-10 µg, ATGP2496-20 µg, ATGP2496-50 µg, ATGP2496-100 µg, ATGP2496-250 µg, ATGP2496-500 µg, ATGP2496-1 mg, ATGP2496-10, ATGP2496-20, ATGP2496-50, ATGP2496-100, ATGP2496-250, ATGP2496-500, ATGP2496-1

References & Citations

Dupre N. et al. (2003) Can J Neurol Sci. 30:122-128; ; Liquori CL. et al. (2007) Am J Hum Genet. 80:69-75.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001161407

Uniprot Accession Number

Q9BSQ5

Species

Human

AA Sequence

EVKYLGQ LTSIPGYLNP SSRTEILHFI DNAKRAHQLP GHLTQEHDAV LSLSAYNVKL AWRDGEDIIL RVPIHDIAAV SYVRDDAAHL VVLKTDDSST KVDIKETYEV EASTFCFPES VDVGGASPHS KTISESELSA SATELLQDYM LTLRTKLSSQ EIQQFAALLH EYRNGASIHE FCINLRQLYG DSRKFLLLGL RPFIPEKDSQ HFENFLETIG VKDGRGIITD SFGRHRRALS TTSSSTTNGN RATGSSDDRS APSEGDEWDR MISDISSDIE ALGCSMDQDS A