Recombinant human MLF1IP protein

MLF1IP, as known as centromere, is a specialized chromatin domain, present throughout the cell cycle that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. It plays an important role in the correct PLK1 localization to the mitotic kinetochores. It is a scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. It also involved in transcriptional repression. Recombinant human MLF1IP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Centromere protein u, CENP50, CENPu, CENPu50, KLIP1, PBIP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

33.7 kDa (295aa)

Additionnal Information

MLF1IP, Centromere protein u, CENP50, CENPu, CENPu50, KLIP1, PBIP1, ATGP2495-10 µg, ATGP2495-20 µg, ATGP2495-50 µg, ATGP2495-100 µg, ATGP2495-250 µg, ATGP2495-500 µg, ATGP2495-1 mg, ATGP2495-10, ATGP2495-20, ATGP2495-50, ATGP2495-100, ATGP2495-250, ATGP2495-500, ATGP2495-1

References & Citations

Foltz DR. et al. (2006) Nat Cell Biol. 8:458-469.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Cancer

NCBI Accession Number

NP_078905

Uniprot Accession Number

Q71F23

Species

Human

AA Sequence

TRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG