Recombinant human CUEDC1 protein
CuEDC1 contains 1 CuE domain. The CuE domain is structurally related to the ubiquitin-binding uBA domain and has been reported to exist as a domain-swapped dimer that makes additional contacts with ubiquitin, and consequently, binds ubiquitin with higher affinity. The CuE domain is found in proteins with diverse functions including degradation of misfolded proteins in the endoplasmic reticulum and protein sorting. Recombinant human CuEDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
CUE domain-containing protein 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
44.6 kDa (409aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gerhard DS, Wagner L, et al. (2004) . Genome Res. 14 (10B) :2121-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Other
NCBI Accession Number
NP_001258804
Uniprot Accession Number
Q9NWM3
Species
Human
AA Sequence
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