Recombinant human Carbonic Anhydrase 14/CA14 protein
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Bioactivity
Molecular Weight
33.2 kDa (298aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Temperini C., et al. (2006) Chemistry. 12:7057-7066; ; Temperini C., et al. (2006) J. Med. Chem. 49:3019-3027;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Neuroscience
NCBI Accession Number
NP_036245
Uniprot Accession Number
Q9ULX7
Species
Human
AA Sequence
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