Recombinant human Carbonic Anhydrase 14/CA14 protein

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Product Name Alternative

Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Bioactivity

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Molecular Weight

33.2 kDa (298aa) confirmed by MALDI-TOF

Additionnal Information

Carbonate dehydratase 14, Carbonate dehydratase XIV, Carbonic anhydrase 14, Carbonic anhydrase XIV, CAXiV, CA-XIV, CA14, ATGP2485-10 µg, ATGP2485-20 µg, ATGP2485-50 µg, ATGP2485-100 µg, ATGP2485-250 µg, ATGP2485-500 µg, ATGP2485-1 mg, ATGP2485-010, ATGP2485-020, ATGP2485-050, ATGP2485-100, ATGP2485-250, ATGP2485-500, ATGP2485-01M

References & Citations

Temperini C., et al. (2006) Chemistry. 12:7057-7066; ; Temperini C., et al. (2006) J. Med. Chem. 49:3019-3027;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_036245

Uniprot Accession Number

Q9ULX7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSADGGQHW TYEGPHGQDH WPASYPECGN NAQSPIDIQT DSVTFDPDLP ALQPHGYDQP GTEPLDLHNN GHTVQLSLPS TLYLGGLPRK YVAAQLHLHW GQKGSPGGSE HQINSEATFA ELHIVHYDSD SYDSLSEAAE RPQGLAVLGI LIEVGETKNI AYEHILSHLH EVRHKDQKTS VPPFNLRELL PKQLGQYFRY NGSLTTPPCY QSVLWTVFYR RSQISMEQLE KLQGTLFSTE EEPSKLLVQN YRALQPLNQR MVFASFIQAG SSYTTGEM