Recombinant human IGFBP-rp1/IGFBP-7 protein

IGFBP7 is a member of the insulin-like growth factor (IGF) -binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm Recombinant human IGFBP7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Insulin-like growth factor-binding protein 7 isoform 1 precursor, insulin-like growth factor-binding protein 7 isoform 1 precursor, AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.8 kDa (279aa) confirmed by MALDI-TOF

Additionnal Information

IGFBP-rp1, IGFBP7, insulin-like growth factor-binding protein 7 isoform 1 precursor, insulin-like growth factor-binding protein 7 isoform 1 precursor, AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF, ATGP2483-10 µg, ATGP2483-20 µg, ATGP2483-50 µg, ATGP2483-100 µg, ATGP2483-250 µg, ATGP2483-500 µg, ATGP2483-1 mg, ATGP2483-10, ATGP2483-20, ATGP2483-50, ATGP2483-100, ATGP2483-250, ATGP2483-500, ATGP2483-1

References & Citations

Akaogi K., et al (1994) . Biochem. Biophys. Res. Commun. 198:1046-1053; ; Oh Y., et al (1996) . J. Biol. Chem. 271:30322-30325;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Scientific Category

Cancer

NCBI Accession Number

NP_001544

Uniprot Accession Number

Q16270

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSSSSDTCG PCEPASCPPL PPLGCLLGET RDACGCCPMC ARGEGEPCGG GGAGRGYCAP GMECVKSRKR RKGKAGAAAG GPGVSGVCVC KSRYPVCGSD GTTYPSGCQL RAASQRAESR GEKAITQVSK GTCEQGPSIV TPPKDIWNVT GAQVYLSCEV IGIPTPVLIW NKVKRGHYGV QRTELLPGDR DNLAIQTRGG PEKHEVTGWV LVSPLSKEDA GEYECHASNS QGQASASAKI TVVDALHEIP VKKGEGAEL