Recombinant human TAZ/WWTR1 protein

WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

WW domain containing transcription regulator 1, TAZ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.5 kDa (423aa)

Additionnal Information

TAZ, WWTR1, WW domain containing transcription regulator 1, TAZ, ATGP2480-10 µg, ATGP2480-20 µg, ATGP2480-50 µg, ATGP2480-100 µg, ATGP2480-250 µg, ATGP2480-500 µg, ATGP2480-1 mg, ATGP2480-10, ATGP2480-20, ATGP2480-50, ATGP2480-100, ATGP2480-250, ATGP2480-500, ATGP2480-1

References & Citations

Huang, W., et al. (2012) J. Biol. Chem. 287 (31), 26245-26253; ; Pan, J., et al. (2012) Oncol. Rep. 28 (1), 179-185;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_001161752

Uniprot Accession Number

Q9GZV5

Species

Human

AA Sequence

MNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL