Recombinant human TRIP10 protein

Cdc42-interacting protein 4, also known as TRIP10, belongs to the F-BAR family of proteins. These proteins contain an N-terminal, alpha-helical, region that is hydrophobic and considered to be related to the Bin Amphiphysin Rvs (BAR) protein family. Expressed in a variety of tissues, including kidney, brain, liver, lung, heart and pancreas, TRIP10 is required for the Insulin-dependent translocation of Glut4 to the plasma membrane and is essential for the coordination of membrane tubulation with Actin cytoskeletal reorganization during endocytosis. Recombinant human TRIP10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cdc42-interacting protein 4, CIP4, HSTP, STOT, STP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.6 kDa (309aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

TRIP10, Cdc42-interacting protein 4, CIP4, HSTP, STOT, STP, ATGP2477-10 µg, ATGP2477-20 µg, ATGP2477-50 µg, ATGP2477-100 µg, ATGP2477-250 µg, ATGP2477-500 µg, ATGP2477-1 mg, ATGP2477-10, ATGP2477-20, ATGP2477-50, ATGP2477-100, ATGP2477-250, ATGP2477-500, ATGP2477-1

References & Citations

Aspenstrom P., et al. (1997) Curr Biol. 7: 479-487.; ; Holbert S., et al. (2003) Proc Natl Acad Sci uSA. 100: 2712-2717.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_004231

Uniprot Accession Number

Q15642

Species

Human

AA Sequence

DPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKK NKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMA EGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN