Recombinant human RPS20 protein

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene u54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. Recombinant human RPS20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

40S ribosomal protein S20 isoform 1, S20

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18.4 kDa (165aa) confirmed by MALDI-TOF

Additionnal Information

RPS20, 40S ribosomal protein S20 isoform 1, S20, ATGP2469-10 µg, ATGP2469-20 µg, ATGP2469-50 µg, ATGP2469-100 µg, ATGP2469-250 µg, ATGP2469-500 µg, ATGP2469-1 mg, ATGP2469-10, ATGP2469-20, ATGP2469-50, ATGP2469-100, ATGP2469-250, ATGP2469-500, ATGP2469-1

References & Citations

Yoshihama M., et al (2002) . Genome Res. 12:379-390; ; Kenmochi N., et al (1998) . Genome Res. 8:509-523;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001139699

Uniprot Accession Number

P60866

Species

Human

AA Sequence

MAFKDTG KTPVEPEVAI HRIRITLTSR NVKSLEKVCA DLIRGAKEKN LKVKGPVRMP TKTLRITTRK TPCGEGSKTW DRFQMRIHKR LIDLHSPSEI VKQITSISIE PGVELIESTD AEPMDTEGQQ YTLRSVFESP GTCPF