Recombinant human Methionine Aminopeptidase 1D protein

Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31. 1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methionine aminopeptidase 1D mitochondrial precursor, Methionine aminopeptidase 1D, mitochondrial precursor, MAP1D, Metap1l

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.4 kDa (339aa) confirmed by MALDI-TOF

Additionnal Information

Methionine Aminopeptidase1D, Methionine aminopeptidase 1D mitochondrial precursor, Methionine aminopeptidase 1D, mitochondrial precursor, MAP1D, Metap1l, ATGP2467-10 µg, ATGP2467-20 µg, ATGP2467-50 µg, ATGP2467-100 µg, ATGP2467-250 µg, ATGP2467-500 µg, ATGP2467-1 mg, ATGP2467-10, ATGP2467-20, ATGP2467-50, ATGP2467-100, ATGP2467-250, ATGP2467-500, ATGP2467-1

References & Citations

Leszczyniecka M., et al. (2006) Oncogene. 25: 3471-3478.; ; Serero A., et al. (2003) J Biol Chem. 278: 52953-52963.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_954697

Uniprot Accession Number

Q6UB28

Species

Human

AA Sequence

SSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA