Recombinant human RAD51B protein

RAD51B is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Recombinant human RAD51B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

DNA repair protein RAD51 homolog 2 isoform 1, RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

40.6 kDa (373aa)

Additionnal Information

RAD51B, DNA repair protein RAD51 homolog 2 isoform 1, RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, ATGP2465-10 µg, ATGP2465-20 µg, ATGP2465-50 µg, ATGP2465-100 µg, ATGP2465-250 µg, ATGP2465-500 µg, ATGP2465-1 mg, ATGP2465-10, ATGP2465-20, ATGP2465-50, ATGP2465-100, ATGP2465-250, ATGP2465-500, ATGP2465-1

References & Citations

Masson J.Y., Tarsounas M.C. et al. (2001) . Genes Dev. 15:3296-3307

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing , 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002868

Uniprot Accession Number

O15315

Species

Human

AA Sequence

MGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS