Recombinant human NDUFS2 protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial isoform 1 precursor, also known as NDUFS2, is a 463 amino acid protein that is suggested to be required for catalytic activity. Defects in NDuFS2 are the cause of complex I mitochondrial respiratory chain deficiency, which is characterized by a variety of symptoms including liver failure, cardiomyopathy and neurodegeneration. Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. Recombinant human NDUFS2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

NADH:ubiquinone oxidoreductase core subunit S2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial, NADH dehydrogenase (ubiquinone) Fe-S protein 2, NADH-coenzyme Q reductase, Complex I-49kD, CI-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, complex I 49kDa subunit, CI-49

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

46.5 kDa (410aa)

Additionnal Information

NDuFS2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial isoform 1 precursor, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial isoform 1 precursor, CI-49, ATGP2463-10 µg, ATGP2463-20 µg, ATGP2463-50 µg, ATGP2463-100 µg, ATGP2463-250 µg, ATGP2463-500 µg, ATGP2463-1 mg, ATGP2463-10, ATGP2463-20, ATGP2463-50, ATGP2463-100, ATGP2463-250, ATGP2463-500, ATGP2463-1

References & Citations

Loeffen J., et al. (2001) Ann Neurol. 49:195-201.; ; Procaccio V., et al. (1998) Genome. 9:482-484.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_004541

Uniprot Accession Number

O75306

Species

Human

AA Sequence

VKNITLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLHRGTEKL IEYKTYLQAL PYFDRLDYVS MMCNEQAYSL AVEKLLNIRP PPRAQWIRVL FGEITRLLNH IMAVTTHALD LGAMTPFFWL FEEREKMFEF YERVSGARMH AAYIRPGGVH QDLPLGLMDD IYQFSKNFSL RLDELEELLT NNRIWRNRTI DIGVVTAEEA LNYGFSGVML RGSGIQWDLR KTQPYDVYDQ VEFDVPVGSR GDCYDRYLCR VEEMRQSLRI IAQCLNKMPP GEIKVDDAKV SPPKRAEMKT SMESLIHHFK LYTEGYQVPP GATYTAIEAP KGEFGVYLVS DGSSRPYRCK IKAPGFAHLA GLDKMSKGHM LADVVAIIGT QDIVFGEVDR