Recombinant human NSDHL protein

NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Sterol-4-alpha-carboxylate 3-dehydrogenase decarboxylating, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, H105E3, SDR31E1, XAP104

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

35.5 kDa (320aa) confirmed by MALDI-TOF

Additionnal Information

NSDHL, Sterol-4-alpha-carboxylate 3-dehydrogenase decarboxylating, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, H105E3, SDR31E1, XAP104, ATGP2460-10 µg, ATGP2460-20 µg, ATGP2460-50 µg, ATGP2460-100 µg, ATGP2460-250 µg, ATGP2460-500 µg, ATGP2460-1 mg, ATGP2460-10, ATGP2460-20, ATGP2460-50, ATGP2460-100, ATGP2460-250, ATGP2460-500, ATGP2460-1

References & Citations

Morimoto M. et al. (2012) J Mol Histol. 43:95-106; ; McLarren KW. et al. (2010) Am J Hum Genet 87:905-914.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_057006

Uniprot Accession Number

Q15738

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY