Recombinant human RPS4X protein

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS4X is ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y) . The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. Recombinant human RPS4X protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

40S ribosomal protein S4 X isoform X isoform, 40S ribosomal protein S4, X isoform X isoform, Ribosomal protein S4, X-linked, CCG2; DXS306, RPS4, S4, SCAR, SCR10

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

32 kDa (286aa) confirmed by MALDI-TOF

Additionnal Information

RPS4X, 40S ribosomal protein S4 X isoform X isoform, 40S ribosomal protein S4, X isoform X isoform, Ribosomal protein S4, X-linked, CCG2; DXS306, RPS4, S4, SCAR, SCR10, ATGP2458-10 µg, ATGP2458-20 µg, ATGP2458-50 µg, ATGP2458-100 µg, ATGP2458-250 µg, ATGP2458-500 µg, ATGP2458-1 mg, ATGP2458-10, ATGP2458-20, ATGP2458-50, ATGP2458-100, ATGP2458-250, ATGP2458-500, ATGP2458-1

References & Citations

Joeson L., Vikesaa J. et al. (2007), Mol. Cell. Proteomics 6:798-811

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000998

Uniprot Accession Number

P62701

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMARGPKK HLKRVAAPKH WMLDKLTGVF APRPSTGPHK LRECLPLIIF LRNRLKYALT GDEVKKICMQ RFIKIDGKVR TDITYPAGFM DVISIDKTGE NFRLIYDTKG RFAVHRITPE EAKYKLCKVR KIFVGTKGIP HLVTHDARTI RYPDPLIKVN DTIQIDLETG KITDFIKFDT GNLCMVTGGA NLGRIGVITN RERHPGSFDV VHVKDANGNS FATRLSNIFV IGKGNKPWIS LPRGKGIRLT IAEERDKRLA AKQSSG