Recombinant human NRAGE protein

MAGED1, as known as Melanoma-associated antigen D1, is expressed in almost all normal adult tissues. It has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. It has been involved in Prader-Willi syndrome, which includes hyperphagia, repetitive and compulsive behaviors, and cognitive impairment. Recombinant human MAGED1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Melanoma-associated antigen D1 isoform a, DLXIN-1, NRAGE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

31.7 kDa (280aa) confirmed by MALDI-TOF

Additionnal Information

NRAGE, Melanoma-associated antigen D1 isoform a, DLXIN-1, NRAGE, ATGP2455-10 µg, ATGP2455-20 µg, ATGP2455-50 µg, ATGP2455-100 µg, ATGP2455-250 µg, ATGP2455-500 µg, ATGP2455-1 mg, ATGP2455-10, ATGP2455-20, ATGP2455-50, ATGP2455-100, ATGP2455-250, ATGP2455-500, ATGP2455-1

References & Citations

Dombret C. et al. (2012) Hum Mol Genet. 21:4703-4717; ; Mouri , . et al. (2012) J Neurosci. 32:4562-4580.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_001005333

Uniprot Accession Number

Q9Y5V3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP