Recombinant human DCI/ECI1 protein

ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Enoyl-CoA delta isomerase 1, DCI, 3,2-trans-enoyl-CoA isomerase, Delta (3), Delta (2) -enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.1 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

ECI1, enoyl-CoA delta isomerase 1, DCI, ATGP2445-10 µg, ATGP2445-20 µg, ATGP2445-50 µg, ATGP2445-100 µg, ATGP2445-250 µg, ATGP2445-500 µg, ATGP2445-1 mg, ATGP2445-10, ATGP2445-20, ATGP2445-50, ATGP2445-100, ATGP2445-250, ATGP2445-500, ATGP2445-1

References & Citations

Janssen u., et al (1994) . Genomics 23:223-228; ; Takahashi Y., et al (1994) . Biochem. J. 304:849-852

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_001910

Uniprot Accession Number

P42126

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG