Recombinant human FAM84B/LRATD2 protein
FAM84B, as known as Family with sequence similarity 84, member B, coprecipitated with a downstream effector of RAS, CRAF. Binding of FAM83B with CRAF disrupted CRAF/14-3-3 interactions and increased CRAF membrane localization, resulting in elevated MAPK and mammalian target of rapamycin (mTOR) signaling. It is an oncogene and potentially represents a new target for therapeutic intervention. Recombinant human FAM84B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
36.9 kDa (333aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kraja AT. et al. (2013) Lipids. 48:155-165; ; Camps J. et al. (2009) Genes Chromosomes Cancer. 48:1002-1017.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl,1mM DTT, 0.1mM PMSF
Scientific Category
Neuroscience
NCBI Accession Number
NP_777571
Uniprot Accession Number
Q96KN1
Species
Human
AA Sequence
Frequently Asked Questions
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