Recombinant human FAM84B/LRATD2 protein

FAM84B, as known as Family with sequence similarity 84, member B, coprecipitated with a downstream effector of RAS, CRAF. Binding of FAM83B with CRAF disrupted CRAF/14-3-3 interactions and increased CRAF membrane localization, resulting in elevated MAPK and mammalian target of rapamycin (mTOR) signaling. It is an oncogene and potentially represents a new target for therapeutic intervention. Recombinant human FAM84B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

LRAT domain containing 2, Family with sequence similarity 84 member B, BCMP101, NSE2, Breast cancer membrane-associated protein 101, Neurological/sensory 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.9 kDa (333aa) confirmed by MALDI-TOF

Additionnal Information

FAM84B, Family with sequence similarity 84 member B, Family with sequence similarity 84, member B, BCMP101, NSE2, ATGP2442-10 µg, ATGP2442-20 µg, ATGP2442-50 µg, ATGP2442-100 µg, ATGP2442-250 µg, ATGP2442-500 µg, ATGP2442-1 mg, ATGP2442-10, ATGP2442-20, ATGP2442-50, ATGP2442-100, ATGP2442-250, ATGP2442-500, ATGP2442-1

References & Citations

Kraja AT. et al. (2013) Lipids. 48:155-165; ; Camps J. et al. (2009) Genes Chromosomes Cancer. 48:1002-1017.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_777571

Uniprot Accession Number

Q96KN1

Species

Human

AA Sequence

MGNQVEK LTHLSYKEVP TADPTGVDRD DGPRIGVSYI FSNDDEDVEP QPPPQGPDGG GLPDGGDGPP PPQPQPYDPR LHEVECSVFY RDECIYQKSF APGSAALSTY TPENLLNKCK PGDLVEFVSQ AQYPHWAVYV GNFQVVHLHR LEVINSFLTD ASQGRRGRVV NDLYRYKPLS SSAVVRNALA HVGAKERELS WRNSESFAAW CRYGKREFKI GGELRIGKQP YRLQIQLSAQ RSHTLEFQSL EDLIMEKRRN DQIGRAAVLQ ELATHLHPAE PEEGDSNVAR TTPPPGRPPA PSSEEEDGEA VAH