Recombinant human BEND6 protein
BEND6, as known as BEN domain-containing protein 6, is a neural BEN-solo factor that shares many functional attributes with Drosophila Insensitive, a co-repressor for the Drosophila CSL factor. This protein binds the mammalian CSL protein CBF1 and antagonizes Notch-dependent target activation. In addition, its association with Notch- and CBF1-regulated enhancers is promoted by CBF1 and antagonized by activated Notch. In utero electroporation experiments showed that ectopic BEND6 inhibited Notch-mediated self-renewal of neocortical neural stem cells and promoted neurogenesis. Recombinant human BEND6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
BEN domain-containing protein 6, bA203B9.1, C6orf65
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.6 kDa (302aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Dai Q. et al. (2013) Development.. 140:1892-1902; ; Chu J. et al. (2004) J Biol Chem. 279:12337-12345.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Scientific Category
Other
NCBI Accession Number
NP_689944
Uniprot Accession Number
Q5SZJ8
Species
Human
AA Sequence
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