Recombinant human SHFM1 protein
SHFM1, as known as 26S proteasome complex subunit DSS1, has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. Recombinant human SHFM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
26S proteasome complex subunit DSS1, DSS1, ECD, SEM1, SHFD1, Shfdg1, SHSF1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
10.7 kDa (93aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Bonache S. et al. (2013) J Cancer Res Clin Oncol. 139:529-32; ; Ma YY. et al. (2013) Carcinogenesis. 34:68-78.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_006295
Uniprot Accession Number
P60896
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSEKKQP VDLGLLEEDD EFEEFPAEDW AGLDEDEDAH VWEDNWDDDN VEDDFSNQLR AELEKHGYKM ETS
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