Recombinant human Dynactin Subunit 2/DCTN2 protein

DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Dynactin subunit 2 isoform 3, Dynactin 2 (p50), DCTN50, DYNAMITIN, RBP50

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

46.6 kDa (424aa) confirmed by MALDI-TOF

Additionnal Information

Dynactin Subunit2, DCTN2, Dynactin subunit 2 isoform 3, Dynactin 2 (p50), DCTN50, DYNAMITIN, RBP50, ATGP2439-10 µg, ATGP2439-20 µg, ATGP2439-50 µg, ATGP2439-100 µg, ATGP2439-250 µg, ATGP2439-500 µg, ATGP2439-1 mg, ATGP2439-10, ATGP2439-20, ATGP2439-50, ATGP2439-100, ATGP2439-250, ATGP2439-500, ATGP2439-1

References & Citations

Echeverri C.J., Paschal B.M. et al. (1996), J. Cell Biol. 132:617-633

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001248342

Uniprot Accession Number

Q13561

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEE LTSTSVEHII VNPNAAYDKF KDKRVGTKGL DFSDRIGKTK RTGYESGEYE MLGEGLGVKE TPQQKYQRLL HEVQELTTEV EKIKTTVKES ATEEKLTPVL LAKQLAALKQ QLVASHLEKL LGPDAAINLT DPDGALAKRL LLQLEATKNS KGGSGGKTTG TPPDSSLVTY ELHSRPEQDK FSQAAKVAEL EKRLTELETA VRCDQDAQNP LSAGLQGACL METVELLQAK VSALDLAVLD QVEARLQSVL GKVNEIAKHK ASVEDADTQS KVHQLYETIQ RWSPIASTLP ELVQRLVTIK QLHEQAMQFG QLLTHLDTTQ QMIANSLKDN TTLLTQVQTT MRENLATVEG NFASIDERMK KLGK