Recombinant human ACOT11 protein

BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Recombinant human BFIT protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

Thioesterase adipose associated isoform BFIT2, Acyl-CoA thioesterase 11, THEA, STASTARD14, BFIT, KIAA0707, BFIT1, THEM1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.9 kDa (268aa)

Additionnal Information

BFIT, Thioesterase adipose associated isoform BFIT2, Thioesterase, adipose associated isoform BFIT2, ATGP2436-10 µg, ATGP2436-20 µg, ATGP2436-50 µg, ATGP2436-100 µg, ATGP2436-250 µg, ATGP2436-500 µg, ATGP2436-1 mg, ATGP2436-10, ATGP2436-20, ATGP2436-50, ATGP2436-100, ATGP2436-250, ATGP2436-500, ATGP2436-1

References & Citations

Thorsell AG, Lee WH., et al. (2011) Plos One 6 p.19521

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_671517

Uniprot Accession Number

Q8WXI4

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSNRTS RKSALRAGND SAMADGEGYR NPTEVQMSQL VLPCHTNQRG ELSVGQLLKW IDTTACLSAE RHAGCPCVTA SMDDIYFEHT ISVGQVVNIK AKVNRAFNSS MEVGIQVASE DLCSEKQWNV CKALATFVAR REITKVKLKQ ITPRTEEEKM EHSVAAERRR MRLVYADTIK DLLANCAIQG DLESRDCSRM VPAEKTRVES VELVLPPHAN HQGNTFGGQI MAWMENVA