Recombinant human Sirtuin 5/SIRT5 protein

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants Recombinant human SIRT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NAD-dependent protein deacylase sirtuin-5 mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.5 kDa (300aa) confirmed by MALDI-TOF

Additionnal Information

Sirtuin5, SIRT5, sirtuin 5, SIR2L5, ATGP2434-10 µg, ATGP2434-20 µg, ATGP2434-50 µg, ATGP2434-100 µg, ATGP2434-250 µg, ATGP2434-500 µg, ATGP2434-1 mg, ATGP2434-10, ATGP2434-20, ATGP2434-50, ATGP2434-100, ATGP2434-250, ATGP2434-500, ATGP2434-1

References & Citations

Zhou, Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314; ; Fischer, F., et al. (2012) PLoS ONE 7 (9), E45098

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_036373.1

Uniprot Accession Number

Q9NXA8

Species

Human

AA Sequence

ARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS